Optimization of osmotic pump controlled release tablets of liensinine solid dispersion by central composite design-response surface methodology / 中草药

Objective: To optimize the prescription of liensinine solid dispersion osmotic pump controlled release tablet and research its release characteristics in vitro. Methods: The cumulative release percent in 2, 6, and 12 h and the linear correlation coefficient of cumulative release curve were taken as evaluation indexes to select the optimal prescription by using the central composite design- response surface methodology (CCD-RSM). The main factors of influence on drug release, which were the dosage of NaCl and PEG 400, and coating agent thickness. Results: The optimal prescription for liensinine solid dispersion osmotic pump controlled release tablet were as follows: NaCl dose was 166.0 mg, PEG 400 content was 80.5%, and the coating weight gain was 3.5%. Conclusion: The prescription optimization model of liensinine solid dispersion osmotic pump controlled release tablet is optimized by CCD-RSM, and it is proved to follow zero-order release kinetics.

Effect of hypoxia inducible factor-1alpha on cells of hair follicle / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the feasibility of stable transfection of human hypoxia inducible factor-1alpha (HIF-1alpha) gene into fibroblasts cells and the effects of supernatant from the transfected cell culture on hair follicle cells.</p><p><b>METHODS</b>PcDNA-HIF1alpha was stably transfected into fibroblasts cells with lipofectamine 2000. Expression of HIF-1alpha was observed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The supernatant was obtained to detect the expression of vascular endothelial growth factor (VEGF) by ELISA. The mRNA expression of basic fibroblast growth factor (bFGF) was detected by RT-PCR. MTT was used to detect the activity of fibroblasts cells and dermal sheath cells added with supernatant.</p><p><b>RESULTS</b>PcDNA-HIF1alpha was successfully transfected into fibroblasts cells. HIF-1alpha could be detected by RT-PCR and Western blot. The expression of VEGF in the supernatant of cells transfected with PcDNA-HIF1alpha was detected. The mRNA expression of bFGF was significantly higher than in the control group (P < 0.01). MTT showed the activity of cells added with supernatant was enhanced (P < 0.05).</p><p><b>CONCLUSION</b>PcDNA-HIF1alpha can stably transfected into fibroblasts cells, and the expressed HIF-1alpha induces the expression of VEGF and bFGF, and the expressed VEGF enhances the activity of cells.</p>